Creating analytical tools that support the advancement of knowledge can often come about through the integration of existing information and techniques, theoretical and practical, directed toward a ...

Protein purification can be a challenging process. Since proteins are expressed in biological systems such as cells, the protein must be purified from a complex mixture. Generic column-based ...

Affinity chromatography theoretically does just that—a ligand that specifically interacts with the target protein is immobilized on a chromatography matrix; the target protein binds to the column, and ...

Effective and efficient protein purification processes are essential in drug discovery and development. Affinity fusion tags are commonly used in protein purification due to their ability to enable ...

Although affinity tag methods for protein purification have greatly accelerated research in laboratories throughout the world, the presence of the tag on the purified target can modify the target and ...

Protein yield and ease of purification are two important criteria in choosing a system for the expression/ purification of recombinant proteins. The IMPACT (Intein Mediated Purification with an ...

Labile, poorly expressed, or poorly soluble proteins paose many difficulties in isolation and purification. These difficulties can be overcome, however, by various means. One approach is to modify ...

Purification by affinity chromatography on immobilized calmodulin is suitable for recombinant proteins expressed in bacteria because endogenous bacterial calmodulin-binding proteins are unknown.