The COVID-19 pandemic led to many changes. Among them is universal familiarity with a molecular diagnostic technique that was largely unknown outside clinical use. The polymerase chain reaction — or ...
The development of the polymerase chain reaction (PCR) in 1983 by Kary Mullis and coworkers revolutionized molecular biology, allowing scientists to amplify DNA sequences for various applications. 1 ...
Designing reproducible PCR assays involves optimizing multiple moving targets, from standardizing each component in sometimes minute reaction volumes to planning ahead to ensure long-term and secure ...
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