Broad staining of myelin sheets and axonal processes in white matter throughout the entire brain. Manually selected location of the protein positivity, observed by immunofluorescence staining in mouse brain. Soma and myelin sheet in oligodendrocytes.

Nov 28, 2017 · Here, we demonstrate using four different rodent models of both neonatal brain injury and adult stroke that staining brain sections for myelin-basic-protein (MBP) enables highly detailed...

Mar 4, 2022 · Here, we study the integrity of myelinated tracts after experimentally preventing the formation of new myelin in the CNS of adult mice, using an inducible Mbp null allele. Oligodendrocytes...

Mar 19, 2018 · Using high-resolution, intravital label-free and fluorescence optical imaging in mouse cortex, we demonstrate lifelong oligodendrocyte generation occurring in parallel with structural...

In the present report we describe a newly developed compound, (E,E)-1,4-b is(4′-aminostyryl)-2-d imethoxy-b enzene (BDB), which is a brain-permeable myelin stain. BDB is a fluorescent stilbenzene derivative that is selectively retained in white matter by binding to myelin.

Feb 1, 2017 · DTI-derived measures of fractional anisotropy (FA), radial diffusivity (RD), and axial diffusivity (AD) were compared to antibody-based labeling of myelin basic protein (MBP), as measured by fluorescence microscopy. We used a customized CLARITY hydrogel solution to facilitate whole brain tissue clearing and subsequent immunolabeling.

Nov 1, 2024 · Brain permeability is an important index to evaluate whether a probe has excellent application value. Compound 32 (40 mg/kg) was injected into mice through the tail vein, it was able to enter the mouse brain and stain the myelin in situ. Compared with MBP antibody or Black-Gold II, it also showed stronger myelin staining ability (Fig. 3 B-C).

We herein developed a near-infrared aggregation-induced emission-active probe, PM-ML, for high-performance myelin imaging. PM-ML could specifically image myelinated fibers in teased sciatic nerves and mouse brain tissues with high contrast, good photostability, and deep penetration depth.

In the present report we describe a newly developed compound, (E,E)-1,4- bis (4′-aminostyryl)-2- di methoxy- b enzene (BDB), which is a brain-permeable myelin stain. BDB is a fluorescent stilbenzene derivative that is selectively retained in white matter by binding to myelin.

Feb 15, 1999 · Recombinant AAV vectors carrying either the myelin basic protein (MBP) promoter (rAAV-MBP-GFP) or the cytomegalovirus (CMV) immediate early promoter (rAAV-CMV-GFP) were semistereotactically injected into the brain of C57BL/6J mice.